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KMID : 0381120220440070867
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Genes and Genomics
2022 Volume.44 No. 7 p.867 ~ p.878
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Berberine protects against palmitate induced beta cell injury via promoting mitophagy
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Li Mo
She Jiang
Ma Louyan
Ma Li
Ma Xiaorui
Zhai Jiajia
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Abstract
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Background: Destruction of pancreatic beta cells is the most typical characteristic of diabetes.
Objective: We aimed to evaluate the effect of berberine (BBR), a bioactive isoquinoline derivative alkaloid, on beta cell injury.
Methods: Rodent pancreatic beta cell line INS-1 was treated with 0.5 mM palmitate (PA) for 24 h to establish an in vitro beta cell injury model.
Results: BBR at 5 μM promoted cell viability, inhibited cell apoptosis and enhanced insulin secretion in PA-induced INS-1 cells. BBR treatment also suppressed PA-induced oxidative stress in INS-1 cells, as evidenced by the decreased ROS production and increased activities of antioxidant enzymes. In addition, suppressed ATP production and reduced mitochondrial membrane potential were restored by BBR in PA-treated INS-1 cells. It was further determined that BBR affected the expressions of mitophagy-associated proteins, suggesting that BBR promoted mitophagy in PA-exposed INS-1 cells. Meanwhile, we found that BBR facilitated nuclear expression and DNA-binding activity of Nrf2, an antioxidative protein that can regulate mitophagy. Finally, a rescue experiment was performed and the results demonstrated that the effect of BBR on cell viability, apoptosis and mitochondrial function in PA-induced INS-1 cells were cancelled by PINK1 knockdown.
Conclusions: BBR protects islet β cells from PA-induced injury, and this protective effect may be achieved by regulating mitophagy. The present study may provide a novel therapeutic strategy for β cell injury in diabetes mellitus.
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KEYWORD
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Berberine, Islet beta cells, Antioxidant, Mitophagy
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